Imaging Core Equipment Photos

4D-Nucleofector Core X/Y (Lonza).  The X unit supports nucleofection of various cell numbers in different formats and it is used to transfect cells in suspension. The Y unit enables transfection of adherent mammalian cells in 24-well plates. Cells can be transfected with either DNA or RNA. Information about the 4D-Nucleofector is available on the company’s website.
 

Nikon T1-E microscope with A1 confocal and STORM super-resolution modules. The system allows imaging of live or fixed cells and tissues labeled with fluorophores using conventional or confocal microscopy. In addition, the system is equipped to perform a super-resolution microscopy, specifically STochastic Optical Reconstruction Microscopy (STORM). Super-resolution microscopy allows a precise localization of a fluorophore, which is beyond the diffraction limit of light. Super-resolution microscopy achieves resolution ten times greater than conventional optical microscopy. The system is also equipped to perform spectral imaging experiments and other state-of-the-art fluorescent optical approaches (TIRF, FRET, FRAP). A high temporal and spatial resolution EMCCD camera is attached to the system. The system is equipped with a stage top environmental chamber for prolonged observation of living cells.

Zeiss Axiovert 200 M microscope. This microscope is one of the most popular microscopes in the Image Analysis Core Facility. The microscope is inverted, fully motorized and equipped with 10x, 20x and 63x objectives for a wide filed fluorescence microscopy. Color and monochrome Axiocam CCD cameras are attached to the microscope, allowing capture of colored samples (e.g. H&E, peroxidase stained samples) or low-intensity florescent samples (e.g. YFP, GFP, RFP and DAPI), respectively. The objectives and filters also permit acquisition of phase contrast and differential interference contrast (DIC) images. The microscope stage is equipped with a holder that accommodates various sizes cell culture dishes and plates, allowing a quick examination of transfection efficiency.

Zeiss Axio Imager M2 microscope for stereoscopic analysis. This system is fully motorized and configured for bright field and multi-channel fluorescent imaging. The microscope is equipped with x, y, z motorized stage and Zeiss Apotome system to create focal optical sections through your thick specimens using structured illumination. Images are acquired with a low noise CMOS chip 2048 x 2048 pixels monochrome digital camera (ORCA-Flash4.0, Hamamatsu). It works with Stereo Investigator, Neurolucida, and deconvolution software (MBF Bioscience).

Workstation for data analysis. Several computers are available for off-line analysis of digital images created in the facility with all necessary software for image data analysis.